Detection of fatty acids in the cell membrane of Escherichia hermanii from different clinical samples in Mosul city, Iraq

Samaa Mones Saeed^✉and  Aws Ibrahim Sulaiman

Department of Biology, College of Science, University of Mosul, Mosul, Iraq

Received: Feb 2, 2023/ Revised: Mar 5, 2023/Accepted: Mar 7, 2023

(✉) Corresponding Author: samaalhamdany@gmail.com

Abstract

Single dimension gas chromatography (GC) is frequently employed in conjunction with mass spectrometry for the analysis of a wide range of lipid samples from a variety of sources (MS). The result of the analysis of membrane lipid of E. hermanii by this technique is that most of the lipids are (Ricinoleic.acid:12420143, Glycerin:6814721 ,2 ,4 Do decadienal, E, E:5915936, Chloromethyl 6-chloroundecanoate:4761461, Ethyl 5-methylhexanonate:3468461, Glycerin:3272473, propane,2-fluoro-2-methyl :3146268, p-Dioxane-2,3-diol:2993346, N, N-Dimethyl-O-(1-methyl-butyl)-hydroxyl:2378662,1-5-Ethyl-tetrahydrofuran-2-yl-3,3-dimethyl:2305938) respectively.

Keywords: Escherichia hermanii, Fatty acid, cell membrane GC-MS analysis

References

Brenner, D., Davis, B., Steigerwalt, A., Riddle, C., McWhorter, A., Allen, S., Farmer III, J., Saitoh, Y. and Fanning, G. (1982). Atypical bio groups of 617 Escherichia coli found in clinical specimens and description of Escherichia hermannii. nov.” Journal of clinical microbiology 15(4): 703-713.

Dawyndt, P., Vancanneyt, M., Snauwaert, C., De Baets, B., De Meyer, H. and Swings, J. (2006). Mining fatty acid databases for detection of novel compounds in aerobic bacteria. Journal of microbiological methods. 66(3): 410-433.‏

Ghazi, M. G. M., Lee, L. C., Sino, H. and Halim, M. I. A. (2022). Review of Contemporary Chemometric Strategies Applied on preparing GC-MS data in forensic analysis. Microchemical Journal. 107732.‏

Kaewpoowat, Q., Permpalung, N. and Sentochnik, D. (2013). Emerging Escherichia pathogen. J Clin Microbiol 51(8):2785-2786.

Poulou, A., Dimitroulia, E., Markou, F. and Tsakris A. (2008) Escherichia hermannii as the sole isolate from a patient with purulent conjunctivitis. Journal of Clinical Microbiology. 46(11):3848–49.

Rice, E. W., Johnson, C. H., Wild, D. K. and Reasoner, D. J. (1992). Survival of Escherichia coli O157: H7 in drinking water associated with a waterborne disease outbreak of hemorrhagic colitis. Letters in applied microbiology, 15(2), 38-40.‏

Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids.‏

Savych, A., Marchyshyn, S., Mosula, L., Bilyk, O., Humeniuk, I. and Davidenko, A. (2022). Analysis of amino acids content in the plant components of the antidiabetic herbal mixture by GC-MS. Pharmacia. 69, 69.‏

Tong, Y. Q., Xin. B. and Sun, S. Q. (2014) Pyelonephritis Caused Solely by Escherichia hermanii. Jundishapur Journal of Microbiology.7(5):18138.

How to cite this article

Saeed, S. M. and Sulaiman, A. I. (2023). Detection of fatty acids in the cell membrane of Escherichia hermanii from different clinical samples in Mosul city, Iraq. Microbial Science Archives, Vol. 3(1), 18-23. https://doi.org/10.47587/MSA.2023.3104

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