Arshed H. Yaseen✉ and Laith A. Yaaqoob
Department of Biotechnology, College of Science, Baghdad University, Baghdad, Iraq
Received: Feb 21, 2023/ Revised: Mar 10, 2023/Accepted: Mar 14, 2023
(✉) Corresponding Author: arshed.h87@uosamara.edu.iq
Abstract
Multi-drug resistant bacteria Acinetobacter baumannii developed as a result of improperly used antibiotics, which are hard to treat and led to serious health problems all over the world. Therefore, it has become necessary to find some alternative ways to overcome this problem and secure human health. In this study, cobalt oxide nanoparticles were biosynthesized using blood serum from individuals with high blood levels as a reducing and stabilizing agent. Additionally, research on the therapeutic uses of the created nanoparticles’ antibacterial activity against the human pathogenic bacteria A. baumannii is being done. Co oxide NPs formation was characterized and confirmed using UV-VIS, atomic force microscopy (AFM), X-ray diffractometer (XRD), and transmission electron microscopy (TEM). Biosynthesized Cobalt oxide NPs appeared in polycrystalline structure with an average diameter of 43.45nm. Bacterial isolates were identified using morphological, gram stains, and biochemical tests for more confirmation Vitek-2 system was used also and the results showed that out of 172 clinical samples, 91 isolates were identified as A. baumannii. Antibiotic Susceptibility test was done against 12 various antibiotics to determine the MDR isolate for further study. Anti-bacterial activity test against MDR A. baumannii was carried out using different concentrations of Co oxide NPs (1.25, 2.5, 5,10, and 20 mg/mL). The biological activity results of biosynthesized Co oxide NPs showed that the maximum inhibition concentration was 20mg/ml with a zone diameter of 22 mm while the minimum inhibition concentration (MIC) was 5 mg/ml with a 10mm zone diameter.
Keywords: Cobalt Oxide, Nanoparticle Characterizations, Biosynthesis, Serum, A. baumannii, Antibacterial Activity
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How to cite this article
Yaseen, A. H. and Yaaqoob, L. A. (2023). Biosynthesis of cobalt oxide nanoparticles using blood serum as reducing and stabilizing agent for therapeutic use against multi-drug resistant Acinetobacter baumannii. Microbial Science Archives, Vol. 3(1), 24-31. https://doi.org/10.47587/MSA.2023.3105
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