Diop Abdou¹✉
, Ndiaye Babacar¹, Serigne Mourtada Mbacké SOW³ , Diallo Thierno Abdoulaye¹, Mahou Chantal¹ and Seck Abdoulaye¹,²
¹Medical Biology Laboratory, Pasteur Institute of Dakar, Senegal
²Cheikh Anta Diop University of Dakar, Senegal
³Kaffrine National Hospital Centre, Senegal
Received: Jul 5, 2023/ Revised: Aug 28, 2023/Accepted: Sept 1, 2023
(✉) Corresponding Author: diopabdou03@yahoo.fr
Abstract
Haemophilus influenzae, a Gram-negative coccobacillus unique to humans, is known to cause a substantial number of respiratory tract infections, along with severe invasive conditions like meningitis and septicemia. This study aimed to ascertain the antibiotic susceptibility patterns of H. influenzae strains isolated from the Pasteur Institute Medical Laboratory in Dakar.A retrospective study was carried out between January 2018 and February 2022 on strains isolated from respiratory tract samples (sputum, BAL). Strains were isolated after culture on Chocolate PVX agar and incubated at 35°C in 5% CO2 for 24 to 48 hours. Haemophilus influenzae-like colonies were identified by conventional methods including requirement tests for haemin (factor X) and NAD (factor V). Biochemical identification was performed using API 20 NH biochemical strips (BioMerieux, France). Disk diffusion test or Kirby-Bauer method had been used to perform antibiotic susceptibility testing on Chocolate PVX media. 132 strains of H. influenzae were isolated. 56% of the strains were from females and 44% from males with a mean age of 44 years. The most represented age groups were [40-59] and [³60] with 31% each. Of the strains, 126 (95%) were from sputum and 6 (5%) from bronchoalveolar lavage (BAL). Resistance rates of H. influenzae isolates to ampicillin, amoxicillin+clavulanic acid, cefepime, cefotaxime, chloramphenicol, ciprofloxacin, meropenem and tetracycline were 24.7%, 22.2%, 72.7%, 9.5%, 8.1%, 8.3% and 22.1%, 22.8% respectively. Antibiotic resistance in H. influenzae requires continued attention with effective detection methods and a rational antibiotic use policy.
Keywords: Haemophilus influenzae, antimicrobial resistance, Biochemical identification, Resistance rates
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How to cite this article
Abdou, D. Babacar, N., SOW, S.M.M., Abdoulaye, D.T., Chantal, M. & Abdoulaye, S. (2023). Antibiotic susceptibility profiles of Haemophilus influenzae isolates collected in Dakar between 2018 and 2022. Microbial Science Archives, Vol. 3(3), 120-124. https://doi.org/10.47587/MSA.2023.3307
