Abeer Mahmood Faraj

Department of Pharmacy Technologies, Northern Technical University Medical Technical Institute- Mosul, Iraq

 Received: April 2, 2025/ Revised: May 14, 2025/Accepted: May 27, 2025

(✉) Corresponding Author: abeer23mah@gmail.com

Abstract

The precise and quick identification of pathogenic microorganisms remains a critical component of efficient clinical diagnostics and antimicrobial stewardship. Traditional culture-based approaches, while reliable, are time-consuming and may not discover fastidious or slow-growing organisms. In this study, we investigated the diagnostic synergy between 16S rRNA gene sequencing, the molecular gold standard for bacterial identification, and the novel VITAK technology, a fast, culture-independent platform based on metabolic profiling. Our goal was to determine the combined performance of these techniques in recognizing both pure isolates and complicated clinical specimens, such as sputum samples. Studies showed that combining the sequencing of 16S rRNA with VITAK dramatically shortened diagnostic turnaround time, with bacterial identification completed in 30-72 hours, compared to the typical delays of standard methods. VITAK’s high sensitivity, automation, and ease of use enhanced the genotypic precision of 16S rRNA analysis, particularly in detecting polymicrobial illnesses and distinguishing between closely connected species. Furthermore, the combination treatment was effective in a variety of clinical settings, including cases of subacute endocarditis and pneumonia with negative cultures. This study demonstrates the potential of a hybrid diagnostic strategy that combines high-throughput sequencing with quick metabolic detection to increase diagnostic accuracy and timeliness in microbiology laboratories. Future plans include expanding this platform to cover fungal and viral targets, improving automation, and testing the system across a larger patient population. The 16S-VITAK synergy is a promising improvement in next-generation diagnostics, capable of guiding more targeted therapeutics and improving patient outcomes.

Keywords: Vitak, 16S rRAN , Synergy, Diagnosis

References

An, X. L., Wang, J. Y., Pu, Q., Li, H., Pan, T., Li, H. Q., … & Su, J. Q. (2020). High-throughput diagnosis of human pathogens and fecal contamination in marine recreational water. Environmental Research, 190, 109982.

Ashfaq, M. Y., Da’na, D. A., & Al-Ghouti, M. A. (2022). Application of MALDI-TOF MS for identification of environmental bacteria: A review. Journal of environmental management, 305, 114359.

Chen, B., Jiang, Y., Cao, X., Liu, C., Zhang, N., & Shi, D. (2021). Droplet digital PCR as an emerging tool in detecting pathogens nucleic acids in infectious diseases. Clinica chimica acta.

Chen, M., Liu, D., Zi, B., Chen, Y., Liu, D., Du, X., … & Pan, H. (2022). Remarkable synergistic effect in cobalt-iron nitride/alloy nanosheets for robust electrochemical water splitting. Journal of Energy Chemistry, 65, 405-414.

Church, D. L., Cerutti, L., Gürtler, A., Griener, T., Zelazny, A., & Emler, S. (2020). Performance and application of 16S rRNA gene cycle sequencing for routine identification of bacteria in the clinical microbiology laboratory. Clinical microbiology reviews, 33(4), 10-1128.

Ejazi, S. A., Ghosh, S., & Ali, N. (2021). Antibody detection assays for COVID‐19 diagnosis: an early overview. Immunology and cell biology.

Garner, E., Davis, B. C., Milligan, E., Blair, M. F., Keenum, I., Maile-Moskowitz, A., … & Pruden, A. (2021). Next generation sequencing approaches to evaluate water and wastewater quality. Water Research, 194, 116907.

Govender, K. N., Street, T. L., Sanderson, N. D., & Eyre, D. W. (2021). Metagenomic sequencing as a pathogen-agnostic clinical diagnostic tool for infectious diseases: a systematic review and meta-analysis of diagnostic test accuracy studies. Journal of clinical microbiology, 59(9), 10-1128.

Gu, W., Deng, X., Lee, M., Sucu, Y. D., Arevalo, S., Stryke, D., … & Chiu, C. Y. (2021). Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids. Nature medicine, 27(1), 115-124.

Guo, L., Wan, K., Zhu, J., Ye, C., Chabi, K., & Yu, X. (2021). Detection and distribution of vbnc/viable pathogenic bacteria in full-scale drinking water treatment plants. Journal of Hazardous Materials.

Li, N., Cai, Q., Miao, Q., Song, Z., Fang, Y., & Hu, B. (2021). High‐throughput metagenomics for identification of pathogens in the clinical settings. Small methods.

Matsuo, Y., Komiya, S., Yasumizu, Y., Yasuoka, Y., Mizushima, K., Takagi, T., … & Hirota, K. (2021). Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution. BMC microbiology, 21, 1-13.

Miller, S. & Chiu, C. (2022). The role of metagenomics and next-generation sequencing in infectious disease diagnosis. Clinical chemistry.

Mustafa, M. I. & Makhawi, A. M. (2021). SHERLOCK and DETECTR: CRISPR-Cas systems as potential rapid diagnostic tools for emerging infectious diseases. Journal of Clinical Microbiology.

Ruan, Y., Hu, B., Liu, Z., Liu, K., Jiang, H., Li, H., … & Wang, T. (2021). No detection of SARS‐CoV‐2 from urine, expressed prostatic secretions, and semen in 74 recovered COVID‐19 male patients: a perspective and urogenital evaluation. Andrology, 9(1), 99-106.

Szlachta-McGinn, A., Douglass, K. M., Chung, U. Y. R., Jackson, N. J., Nickel, J. C., & Ackerman, A. L. (2022). Molecular diagnostic methods versus conventional urine culture for diagnosis and treatment of urinary tract infection: a systematic review and meta-analysis. European Urology Open Science, 44, 113-124.

Yang, X., Jiang, G., Zhang, Y., Wang, N., Zhang, Y., Wang, X., … & Wei, Z. (2023). MBPD: A multiple bacterial pathogen detection pipeline for One Health practices. Imeta, 2(1), e82.

Zakerska-Banaszak, O., Tomczak, H., Gabryel, M., Baturo, A., Wolko, L., Michalak, M., … & Skrzypczak-Zielinska, M. (2021). Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study. Scientific Reports, 11(1), 2166.

How to cite this article

Faraj, A. M. (2025)16S rRNA and VITAK Synergy: A Breakthrough in Diagnostic Microbiology. Microbial Science Archives, Vol. 5 (2), 4-10. https://doi.org/10.47587/MSA.2025.5201

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