Medoune Ndiaye¹,²*, Mamadou Soumboudou¹, ³ and Serigne Fallou Ndiaye¹
¹Hôpital des Enfants de Diamniadio, Dakar, Sénégal
²Université Cheikh Anta Diop, Dakar, Sénégal
³Université Iba Der Thiam, Thies, Sénégal
Received:Nov 24, 2024/ Revised: Dec 23, 2024/Accepted: Dec 28, 2024
(✉) Corresponding Author: medounebiologie@gmail.com
Abstract
The recurrence of carbapenem-resistant Enterobacteriaceae cases is alarming with potential therapeutic dead-ends that may have serious clinical consequences. Thus, this study aims to perform the modified Hodge test as phenotypic screening for carbapenemase production. This study was conducted at the bacteriology unit of the medical biology laboratory at Diamniadio Children’s Hospital in the Dakar region of Senegal between June 2022 and June 2023. The 17 isolates came from pus samples, swabs, and urine collected from patients in neonatology and surgical hospitalization departments. Strains showing intermediate or resistant zones of 17 mm to 22 mm for imipenem and meropenem were subjected to the Modified Hodge Test, with comparison to MIC. Of the 17 strains, 15 were positive (88%) for carbapenemase production by modified Hodge test. The carbapenemase-producing organisms were Enterobacter spp (40%), Klebsiella pneumoniae (33%), Escherichia coli (20%), Citrobacter freundii (07%). In the absence of advanced technical platforms, the Hodge test is a good tool for confirming carbapenemase production, however some limitations exist and should be complemented by molecular tests.
Keywords: Modified hodge test, Carbapenemase, Antimicrobial resistance
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How to cite this article
Ndiaye, M., Soumboudou, S. and Ndiaye, S.F. (2024). Detection of carbapenemase production in enterobacteriaceae using the modified hodge test. Microbial Science Archives, Vol. 4(4), 35-38. https://doi.org/10.47587/MSA.2024.4404.









