Pratima Verma¹✉, Sumit Kumar Rawat¹, Nitu Mishra¹, Amardeep Rai¹, Shraddha Mishra²
¹Department of Microbiology, Bundelkhand Medical College, Sagar, Madhya Pradesh, India
²Department of Community Medicine, Bundelkhand Medical College, Sagar, Madhya Pradesh, India
Received: May 12, 2023/ Revised: June 8 / Accepted: June 10, 2023
(✉) Corresponding Author: pratimaverma2308@gmail.com
Abstract
To study the seroprevalence in dengue and its correlation with hematological parameters. A retrospective analysis of 718 seropositive cases was done for one year from January 2021-December 2021. The rapid diagnostic test kit showing NS1 Ag, IgM Ab, and IgG Ab reactivity toward dengue was used. The dengue seropositive cases were divided into 7 serogroups (NS1Ag), (IgM Ab), (IgG Ab), (NS1 Ag IgM Ab), (NS1 Ag IgG Ab), (IgM Ab IgG Ab), (NS1Ag IgM Ab IgG Ab). The hematological data were analyzed. And the correlation between serological and hematological parameters was calculated using the ANOVA test. The dengue-positive cases were observed highest between 19-60 years of age (48.7%), along with male predominance and peak during October month (49.72%). The highest Seroprevalence was observed with NS1Ag (59.05%) followed by IgG Ab (22.42%) in outdoor patients and adults, whereas NS1Ag IgM Ab (6.55%) followed by IgM Ab (4.18%) was predominantly seen in indoor patients and children (0-12 yrs). The maximum affected hematological variable were platelet count (thrombocytopenia in 55.8%) and total leucocyte count (leucopenia in 38.0%) having a strong association with NS1Ag positivity followed by lymphocytosis (17.1%) and neutropenia (11.6%) which were in concordance with other studies. Both (NS1Ag and IgM Ab) detection in the active stage has a probable diagnosis of primary dengue infection and IgG detection in secondary infection. Thrombocytopenia and leucopenia are early markers of dengue in view of NS1Ag association. Thus, serological markers together with hemograms help in the early detection, treatment, and ascertaining prognosis of the disease.
Keywords: Dengue, Seroprevalence, Serological Markers, Hematological Parameters.
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How to cite this article
Verma, P., Rawat, S. K., Mishra, N., Rai, A. and Mishra, S. (2023). Evaluation of Utility of Serological Markers Using Rapid Diagnostic Test Kits and Investigating their Correlation with Peripheral Blood Count in a Dengue Fever Outbreak. Microbial Science Archives, Vol. 3(2), 52-57. https://doi.org/10.47587/MSA.2023.3201
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