Hussam Abdul Razzaq Ismail and Rasheed Hameed Hassan
University of Samarra, College of Education, Department of Biology, Iraq
*Corresponding author: hussammicrobio@gmail.com
Abstract
This study was conducted in the laboratories of the Department of Biological Science University of Samarra/College of Education for the period from 1/11/2020 to 10/4/2021 AD. Samples of Lactobacillus casei were collected from milk and various food sources, and they were grown on a selective nutrient medium; De Man Rogosa Sharpe (MRS) was then isolated and diagnosed in the Microbiology laboratory, and two types of pathogenic bacteria were used: Staphylococcus aureus and Klebsiella pneumoniae to determine the effect of bacteriocin on it. The study also included the quantitative detection of the protein of bacterial filtrate produced from Lactobacillus casei, as well as the study of the effect of Bacteriocin on the pathogenic bacterial species under study in three concentrations (1.64, 3.28, 6.56 mg/ml). The bacterial filtrate showed inhibitory activity against the bacterial species under study, which showed sensitivity to bacteriocin, and the inhibitory activity of the bacterial filtrate increased with increasing its concentration, as it gave the concentration (6.56) mg /ml. The highest inhibitory activity against the bacterial species under study was with an inhibitory diameter of (15.1 mm) for S. aureus and an inhibitory diameter of (14.2 mm) for K. pneumoniae. The Minimum Inhibitory Concentration (MIC) of bacteriocin was determined in this study at concentrations (0.21, 0.41, 0.82, 1.64, 3.28, 6.56 mg/ml). The MIC value varied according to the type, source, and concentration of the bacterial filtrate, as well as the type of bacteria. The (MIC) value of S. aureus was (1.64 mg /ml), while the (MIC) value of K . pneumoniae was (3.28 mg/ml).
Keywords Staphylococcus aureus and Klebsiella pneumoniae , Bacteriocin , Lactobacillus casei
References
Al-Rawi, K. M., Abd, A. and Khalaf, M. A. (2000). Design and Analysis of Experiments, 2nd Edition, Ministry of Higher Education and Scientific Research, Dar al Kutub for printing and publishing , University of Mosul , Iraq .
Al-Sahookei, M. M. (1990). Yellow corn production and quality. Ministry of Higher Education and Scientific Research.
AL-Samarraie, M. Q., Omar, M. K., Yaseen, A. H., & Mahmood, M. I. (2019). The wide spread of the gene haeomolysin (Hly) and the adhesion factor (Sfa) in the E. coli isolated from UTI. Journal of Pharmaceutical Sciences and Research, 11(4), 1298-1303.
Chatterjee, R., Singh, D., Tripathi, S., Chauhan, A., Aggarwal, M. L., & Varma, A. (2021). Isolation and Characterization of Multiple Drug Resistant Human Enteric Pathogens from Sewage Water of Delhi. Nature Environment and Pollution Technology, 20(2), 569-578.
Chauhan, A., Chauhan, G., Gupta, P. C., Goyal, P., & Kaushik, P. (2010). In vitro antibacterial evaluation of Anabaena sp. against several clinically significant microflora and HPTLC analysis of its active crude extracts. Indian journal of pharmacology, 42(2), 105.
Chauhan, A., Pandey, V., Chacko, K. M., & Khandal, R. K. (2010). Antibacterial activity of raw and processed honey. Electronic journal of biology, 5(3), 58-66.
Dimopoulous, G. and Falagas, M. E. (2007). Gram negative bacilli resistance issues Touch Briefing. pp.49-51.
Dimri, A. G., Prasad, R., Chauhan, A., Aggarwal, M. L., & Varma, A. (2018). Characterization of soil actinomycete isolate against gram-positive and gram-negative food borne bacteria. IJEP, 38, 1004-1015.
Goyal, P., Chauhan, A., & Kaushik, P. (2010). Assessment of Commiphora wightii (Arn.) Bhandari (Guggul) as potential source for antibacterial agent. Journal of Medicine and Medical Sciences, 1(3), 71-75.
Hammadi, F. G. (2017). Determination of the inhibitory effect of bacteriocin extract from some types of lactic acid bacteria on Helicobacter pylori, PhD thesis, College of Education for pure Science, Tikrit University.
Jasim, S. A., Abdulrazzaq, S. A., & Saleh, R. O. (2020). Virulence Factors of Klebsiella pneumoniae Isolates from Iraqi Patients. Systematic Reviews in Pharmacy, 11(6).
Jawetz, E., Melnick, J. L., Adelberg, E. A., Brooks, G. F., Butel, J. S., & Ornston, L. N. (1991). Medical Microbiology 19 edn.
Kaushik, P., & Chauhan, A. (2008). In vitro antibacterial activity of laboratory grown culture of Spirulina platensis. Indian Journal of Microbiology, 48(3), 348-352.
Kaushik, P., Chauhan, A., Chauhan, G., & Goyal, P. (2008). Evaluation of Nostoc commune for potential antibacterial activity and UV-HPLC analysis of methanol extract. Int J Microbiol, 5(1), 1-5.
Majeed, H. M., Noomi, B. S., & AL-Samarraie, M. Q. (2020). Detection of some virulence factors and antibiotic sensitivity test of enterococcus faecalis isolated from sheep by multiplex pcr. European Journal of Molecular & Clinical Medicine, 7(9), 68-74.
Mustafa, M. A., AL-Samarraie, M. Q. & Ahmed, M. T. (2020). Molecular techniques of viral diagnosis. Science Archives, 1(3), 89-92.
Nayyef, S. H., Salih, N. I., & Mustafa, M. A. (2020). Antimicrobial Activity Of Aloe Vera And Opuntia Ficus Extract Against Certain Pathogenic Bacteria. European Journal of Molecular & Clinical Medicine, 7(9), 422-428.
Ogunbanwo, S. T., Sanni, A. I. & Onilude, A. A. (2003). Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. African Journal of Biotechnology, 2(8), 219-227.
Podschun, R., & Ullmann, U. (1998). Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical microbiology reviews, 11(4), 589-603.
Probst, R., Grevers, G., & Iro, H. (2006). Basic otorhinolaryngologya step-by-step learning guide. Georg Thieme Verlag,5: p. 98-12.
Rashid, T., & Ebringer, A. (2007). Ankylosing spondylitis is linked to Klebsiella—the evidence. Clinical rheumatology, 26(6), 858-864.
Rasool, S. A., Ahmad, A. F. S. H. E. E. N., Khan, S., & Wahab, A. (2003). Plasmid borne antibiotic resistance factors among indigenous Klebsiella. Pak. J. Bot, 35(2), 243-248.
Ray, B. and Daeschel, M. (1992). Food biopreservatives of microbial origin. CRC Press Inc.
Tenover, F. C. (2006). Mechanisms of antimicrobial resistance in bacteria. The American journal of medicine, 119(6), S3-S10.
Wiedemann, N., Frazier, A. E., & Pfanner, N. (2004). The protein import machinery of mitochondria. Journal of Biological Chemistry, 279(15), 14473-14476.
Zacharof, M. P., & Lovitt, R. W. (2012). Bacteriocins produced by lactic acid bacteria a review article. Apcbee Procedia, 2, 50-56.
License Article Metadata
This work is licensed under a Creative Commons Attribution 4.0 International License.
