Hiba Sahib Sadeq1 and Mohammed Qasim Waheeb2

 1Department of Medical Laboratories, College of Health and Medical Technology, Sawa University, Samawah, 66001,

Al-Muthana, Iraq

2Department of Biology, College of Science, Al-Muthanna University, Samawah, 66001, Al-Muthana, Iraq

Received: July 09, 2024/ Revised: Aug 12, 2024/Accepted: Aug 14, 2024

(✉) Corresponding Author: mhmdkas@mu.edu.iq

 Abstract

 The primary focus of this research is to analyze the appearance, characteristics, and relationship between antibiotics and E. coli bacteria isolated from urinary tract infections (UTIs). Conducted at Al-Muthanna Governorate Hospital and educational laboratories, 100 clinical samples were collected from the urine of UTI patients between March 5, 2024, and June 6, 2024, all under the supervision of a specialist physician. The antibiotic susceptibility test revealed that the E. coli bacteria were multi-drug resistant (MDR). All 50 isolates (100%) exhibited antibiotic resistance. Specifically, 47 isolates (94%) were resistant to erythromycin, 49 isolates (98%) to carbenicillin, 44 isolates (88%) to rifampin, 38 isolates (76%) to ceftazidime, 37 isolates (74%) to cefotaxime, 26 isolates (52%) to tetracycline, and 33 isolates (66%) to novobiocin.

Keywords: Phenotypic, Escherichia coli, sensitivity, antibiotics.

References

Abdu, A. Kachallah, M. and Yusuf Bolus, D. (2018). Antibiotic Susceptibility Patterns of UropathogenicEscherichia coli Among Patients with Urinary Tract Infections in a Tertiary Care Hospital in Maiduguri, North Eastern, Nigeria. J. BiosciBiotechnolDiscov. 3: 14-24.

Asadi, S.; Kargar, M.; Solhjoo, K.; Najaf, N. and Ghorbani-Dalini, S. (2014). The Association of Virulence Determinants of UropathogenicEscherichia coli with Antibiotic Resistance. Jundishapur J Microbiol. 7(5): 1-5.

Bhattacharyya, S.; Sarfraz, A.; Ansari, M. A. A. and Jaiswal, N. (2015). Characterization and antibiogram of UropathogenicEscherichia coli from a tertiary care hospital in Eastern India. Int J CurrMicrobiol App Sci. 4(2): 701-705.

Brown, A. E. and Smith, H. R. (2017). Bensonʼs Microbiological Applications, Laboratory Manual in General Microbiology. 14thed. McGraw-Hill Higher Education. New York. 438pp.

CLSI. (2017). Performance Standard for Antimicrobial Susceptibility Testing. 27thed. CSLI Supplement M100.Wayne, PA:Clinical and Laboratory Standards Institute.32-41.

Forbes, B. A., Saham, D. F. and Weissfeld, A. S. (2007). Baily and Scott’s Diagnostic Microbiology. 12thed. Mosby, Inc., an anffilliate of Elsevier, Inc. 1031pp.

Hadi, O. M.; Al-Maliki, A. H.; Al-Zubaidy, M. S. M. and Nihmah, Y. K. (2014). Prevalence of Uropathogenic Escherichia coli in Al-Hashymia District of Babylon Province. JUBPAS. 9(22): 2479-2488.

Hegazy, E. E. Alam El-Din, R. A.; Amin, A. M.; Mahgoub, F. M. and El-Gamal,S. A. (2018). Microbiological Profile of Urinary Tract Infections with special Reference to Antibiotic Susceptibility Pattern of Escherichia coli Isolates. Int J CurrMicrobiol App Sci. 7(2): 911-920.

Hemraj, V., Diksha, S. and Avneet, G. (2013). A Review on Commonly Used Biochemical Test for Bacteria. IJLS. 1(1): 1-7.

Hinthon, W., Pumipuntu, N., Santajit, S., Kulpeanprasit, S., Buranasinsup, S., Sookrung, N., Chaicumpa, W., Aiumurai, P. and Indrawattana, N. (2017). Detection and Drug Resistance Profile of Escherichia coli from Subclinical Mastitis Cows and Water Supply in Dairy Farms in Saraburi Province, Thailand. Peer J. 1-16.

Ibrahim, I. A., Al-Shwaikh, R. M. and Ismaeil, M. I. (2014). Virulence and Antimicrobial Resistance of Escherichia coli Isolated from Tigris River and Children Diarrhea. Infect Drug Resist. 7: 317- 322.

Jawetz, E., Melnick, J. A. and Adelberg, E. A. (2016). Review of Medical Microbiology 27thed McGraw-Hill education, Inc: 851pp. Rights Reserved. 300pp.

Kafilzadeh, F. and Farsimadan, F. (2016). Investigating Multidrug Efflux Pumps in Relation to the Antibiotic Resistance Pattern in Escherichia coli Strains from Patients in Iran. Biomed Res. 27(4): 1130-1135.

Kafilzadeh, F. and Farsimadan, F. (2016). Investigating Multidrug Efflux Pumps in Relation to the Antibiotic Resistance Pattern in Escherichia coli Strains from Patients in Iran. Biomed Res. 27(4): 1130-1135.

Kapoor, J., Saigal, S. and Elongavan, A. (2017). Action and Resistance Mechanisms of Antibiotics: A Guide for Clinicians. J Anaesthesiol Clin Pharmacol. 33(3):300-305.

Levinson, W. (2016). Review of Medical Microbiology and Immunology. 14thed. McGraw-Hill education, Inc. PP 821.

Maleki, D., Jahromy, S. H., Karizi, S. Z. and Eslami, P. (2017). The Prevalence of acrAand acrBGenes Among Multiple-Drug Resistant UropathogenicEscherichiacoliIsolated from Patients with UTI in Milad Hospital, Tehran. Avicenna J ClinMicrobInfec. 4(1): 1-7.

Malekzadegan, Y., Khashei, R., Ebrahim-Saraie, H. S. and Jahanabadi, Z. (2018). Distribution of Virulence Genes and Their Association with Antimicrobial Resistance Among UropathogenicEscherichia coli Isolates from Iranian Patients. BMC Infect Dis. 18(572):1-9.

Paltansing, S. (2015). Antimicrobial Resistance in Enterobacteriaceae: Characterization and Detection. Leiden University. 155pp.

Ramírez-Castillo, F. Y., Moreno‑Flores, A. C., Avelar‑González, F. J., Márquez‑Díaz, F., Harel, J. and Guerrero‑Barrera, A. L. (2018). An Evaluation of Multidrug‑Resistant Escherichia coli Isolates in Urinary Tract Infections from Aguascalientes, Mexico: Cross‑Sectional Study. Ann Clin. MicrobiolAntimicrob. 17(34):1-13.

Sharmin, S., Alamgir, F. Begum, F. and Jaigirdar, K. H. (2010). Use of Chromogenic Agar Media for Identification of Uropathogen. Bangladesh J Med. Microbiol. 4 (1): 18-23.

Suresh, M., Nithya, N., Jayasree, P. and Kumar, M. P. (2016). Detection andPrevalence of Efflux Pump-Mediated Drug Resistance in Clinical Isolates ofMultidrug-Resistant Gram-Negative Bacteria from North Kerala, India. Asian JPharmClin Res. 19(3): 324-327.

Tajbakhsh, E., Ahmadi, P., Abedpour-Dehkordi, E., Arbab-Soleimani, N. and Khamesipour, F. (2016). Biofilm Formation, Antimicrobial Susceptibility, Serogroups and Virulence Genes of Uropathogenic E. Coli. Isolated from Clinical Samples in Iran. Antimicrob Resist Infect Control. 5(11): 1-8.

Terlizzi, M. E., Gribaudo, G. and Maffei, M. E. (2017). UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-Antibiotic Antimicrobial Strategies. Front Microbiol. 8(1566): 1-23. Tille, P.M.

Tille, P. M. (2017). Baily and Scott’s Diagnostic Microbiology. 41thed. Elsevier, Inc. China. 1115pp.

Vandepitte, J., Verhaegen, J., Engbaek, K., Rohner, P., Piot, P. and Heuck, C. C. (2003). Bacteriological Investigations. In: World Health Organization. 2nded. Basic Laboratory Procedures in Clinical Bacteriology, WHO, Geneva. 167pp.

Wanger, A., Chavez, V., Huang, R. S. P., Wahed, A., Actor, J. K. and DasguptaA. (2017). Microbiology and Molecular Diagnosis in Pathology. Elsevier Inc. All

Zaman, S. B., Hussain, M. A., Nye, R., Mehta, V., Mamun, K. T. and Hossain, N. (2017). A Review on Antibiotic Resistance: Alarm Bells are Ringing. Cureus. 9(6): 1-9.

Zowawi, H. M., Harris, P. N. A., Roberts, M. J., Tambyah, P. A., Schembri, M.A., Pezzani, M. D., Williamson, D. A. and Paterson, D. L. (2015). The Emerging Threat of Multidrug-Resistant Gram-Negative Bacteria in Urology. Nat Rev Urol.1-15.

How to cite this article

Sadeq, H. S. and Waheeb, M. Q. (2024). Phenotypic characterization of Escherichia coli bacteria and determination of their sensitivity to antibiotics. Microbial Science Archives, Vol. 4(3), 17-23. https://doi.org/10.47587/MSA.2024.4302

Licence                  Article Metadata

This work is licensed under a Creative Commons Attribution 4.0 International License.

View Details