Sarwa Azeez Khalid¹*, Nada Aziz Khalid², and Rana Aziz Khalid Al bayati³
¹Department of Clinical Laboratory Sciences, College of Pharmacy, University of Tikrit, Tikrit, Iraq
²Department of Biotechnology, College of Applied sciences, University of Samarra, Samarra, Iraq
³Kirkuk Health and Medical Techniques College, Northern Technical University, Iraq
Received: Aug 24, 2024/ Revised: Sep 14, 2024/Accepted: Sep 28, 2024
(✉) Corresponding Author: sarwaazeez@tu.edu.iq
Abstract
Pseudomonas aeruginosa is an important opportunistic bacterium, producing biofilm and two groups of siderophores, the important factors allowing host colonization. The present research aimed to study the siderophores production and biofilm formation among Pseudomonas aeruginosa isolates in patients with different infections in Tikrit City. 30 Pseudomonas aeruginosa isolates were isolated from 80 clinical specimens (for both gender and different age groups) with wound infections, burn infections, otitis infections, and urinary tract infections. Isolates identification based on morphological properties, gram staining, and biochemical tests. Biofilm formation potential of isolates was studied by Congo red agar plate at 370C for 24 h. Also, isolates tested for Siderophores production by using CAS assay. In this study, the results showed that 30 (37.5%) Pseudomonas aeruginosa were isolated from 80 different clinical infections; (6) 20% from burn infections, (10) 33.3% from wound infections, (7)23.3% from UTI, and (7) 23.3% from otitis infections. The current study showed that 10(33.3%) of isolates were biofilm producers while 20 (66.7%) were non-biofilm producers and the maximum of Biofilm production was in wound samples also the study presented that all tested isolates of Pseudomonas aeruginosa were positive for siderophore production. The study showed that Pseudomonas aeruginosa associated with variance patients infections. Biofilm is assessed to be answerable for all human infections and hospital-acquired infections. In this study, the biofilm formation differs between Pseudomonas aeruginosa strains and has been higher in wound infections. Further, all tested isolates were positive for siderophore production, the differences between producers and non-producer bacteria should increase with increasing iron -limitation because production of siderophores is regulated in response to iron limitation.
Keywords: Siderophores Production, Biofilms Formation, Pseudomonas aeruginosa, Clinical Infections.
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How to cite this article
Khalid, S. A., Khalid, N. A. and Al-bayati, R. A. K. (2024). Evaluation siderophores production and biofilms formation among Pseudomonas aeruginosa isolated from clinical infections. Microbial Science Archives, Vol. 4(3), 30-34. https://doi.org/10.47587/MSA.2024.4304.
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