Sarwa Azeez Khalid¹
, Aymen. A. Khalid²✉ and Nada aziz Khalid³
¹Department of Clinical Laboratory Sciences, College of Pharmacy, University of Tikrit, Tikrit, Iraq
²Department of Medical Laboratory Technology, Imam Ja’afar Al-Sadiq University, Kirkuk, Iraq
³Department of Biotechnology, College of Applied Sciences, University of Samarra, Samarra , Iraq
Received: Nov 20, 2023/ Revised: Dec 8, 2023/Accepted: Dec 11, 2023
(✉) Corresponding author Email: aymen.aziz@sadiq.edu.iq
Abstract
The most frequently occurring human bacterial infections are urinary tract infections (UTIs). P. mirabilis is one of the common causes of UTIs in humans with complicated UTIs, the formation of stones in the kidney, and long-term indwelling catheters. This study aims to isolate, and identify Proteusmirabilis from patients with UTIs using traditional methods and 16s rRNA gene sequencing techniques, as well as detect some virulence genes (hpm A and ureR) in Proteus mirabilis by using polymerase chain reaction (PCR). A total of five isolates of Proteus mirabilis were isolated from patients with urinary tract infections in June 2022. Identification of isolates depends on microscopic and cultural characteristics and traditional biochemical tests, finally, isolates are identified by target gene 16s rRNA using PCR technique, in addition, this study investigates the genes responsible for virulence factors such as hpmA and hpmR.In this study laboratory diagnosis and molecular analysis (16s rRNA gene) revealed that the five isolates are Proteus mirabilis also this study shows the presence of the hpmAgenes by PCR techniques in the isolates 100%. Our study has shown that 16s rRNA was a strong eclectic power and an excellent method for the identification of bacteria also these study frequency ofureR and hpmA in P.mirabilis isolated from UTIs and these genes have a manifest role in the infection.
Keywords: Proteus mirabilis, Virulence Genes, Urinary Tract Infection
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How to cite this article
Khalid, S. A., Khalid, A. A. and Khalid, N. A. (2024). Molecular study of hpmA and ureR genes among Proteus mirabilis isolates inpatient with urinary tract infection. Microbial Science Archives, Vol. 4(1) 1-5. https://doi.org/10.47587/MSA.2024.4101
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